Sustained Release of Salicylic Acid for Halting Peri-Implantitis Progression in Healthy and Hyperglycemic Systemic Conditions: A Gottingen Minipig Model.

To develop a peri-implantitis model in a Gottingen minipig and evaluate the effect of local application of salicylic acid poly(anhydride-ester) (SAPAE) on peri-implantitis progression in healthy, metabolic syndrome (MS), and type-2 diabetes mellitus (T2DM) subjects. Eighteen animals were allocated to three groups: (i) control, (ii) MS (diet for obesity induction), and (iii) T2DM (diet plus streptozotocin for T2DM induction). Maxillary and mandible premolars and first molar were extracted. After 3 months of healing, four implants per side were placed in both jaws of each animal. After 2 months, peri-implantitis was induced by plaque formation using silk ligatures. SAPAE polymer was mixed with mineral oil (3.75 mg/µL) and topically applied biweekly for up to 60 days to halt peri-implantitis progression. Periodontal probing was used to assess pocket depth over time, followed by histomorphologic analysis of harvested samples. The adopted protocol resulted in the onset of peri-implantitis, with healthy minipigs taking twice as long to reach the same level of probing depth relative to MS and T2DM subjects (∼3.0 mm), irrespective of jaw. In a qualitative analysis, SAPAE therapy revealed decreased levels of inflammation in the normoglycemic, MS, and T2DM groups. SAPAE application around implants significantly reduced the progression of peri-implantitis after ∼15 days of therapy, with ∼30% lower probing depth for all systemic conditions and similar rates of probing depth increase per week between the control and SAPAE groups. MS and T2DM conditions presented a faster progression of the peri-implant pocket depth. SAPAE treatment reduced peri-implantitis progression in healthy, MS, and T2DM groups.

Correlation between 2D and 3D measurements of cement space in CAD-CAM crowns.

STATEMENT OF PROBLEM: Although the 2D analysis of prosthesis cementation space has been popular, its correlation with volumetric comparison (3D data) of cement space is unclear. PURPOSE: The purpose of this in vitro study was to evaluate the cement space in computer-aided design and computer-aided manufacturing (CAD-CAM) crowns of different materials and correlate 2D measurements of cement space with their corresponding 3D values (volume of cement space) by using microcomputed tomography (µCT) analysis of regions of interest. MATERIAL AND METHODS: Ten molar crowns were milled in lithium disilicate (LD), resin nanoceramic (RN), and zirconia (Z) ceramics. Silicone replicas were produced and used as the analog cement layer and scanned with a desktop X-ray microfocus CT scanner. Twenty-eight slices were evaluated in 3 regions: marginal, axial, and occlusal (n=84 measurement points/specimen). After 3D reconstruction of the cement space, the volume was calculated. Data were statistically evaluated through 2-way ANOVA and Bonferroni test (α=.05). The Pearson correlation test was used to investigate the correlation between the 2D and 3D data. RESULTS: The volumes of the occlusal (LD 10 ±1 mm3; RN 9 ±1 mm3) and axial regions (LD 9 ±2 mm3; RN 8 ±1 mm3) were significantly higher than the volume of the marginal region for LD and RN specimens (LD 6 ±2 mm3; RN 4 ±1 mm3) (both P<.001). For the Z group, the axial region had the highest volume (19 ±2 mm3), followed by the volumes of the occlusal (15 ±1 mm3) and marginal regions (12 ±1 mm3). The Pearson correlation test determined a moderate positive correlation of the marginal area (r=0.606, P<.001) and of the axial region (r=0.588, P<.001). However, a moderate negative correlation was found between volume and thickness of the occlusal area (r=-0.437, P=.016). CONCLUSIONS: Z showed more volume of cement space, as well as thicker cement space than LD and RN. The µCT analysis is an efficient method of analyzing cement thickness and volume in ceramic crowns at the selected regions of interest. A moderate positive correlation was found between the 2D and 3D analyses for the axial and marginal regions of ceramic crowns.

Three-dimensional misfit between Ti-Base abutments and implants evaluated by replica technique.

OBJECTIVE: An important factor affecting the biomechanical behavior of implant-supported reconstructions is the implant-abutment misfit. This study evaluated the misfit between Ti-Base abutments and implants by means of polyvinyl siloxane replica technique using microcomputed tomography (µCT). METHODOLOGY: Volumetric and linear (central and marginal) gaps of four Ti-base abutments (n=10/group): (i) Odontofix LTDA (OD), (ii) Singular Implants (SING), (iii) EFF Dental Components (EFF), and (iv) Control Group (S.I.N implants) compatible with an implant system (Strong SW, S.I.N Implants) were measured using µCT reconstructed polyvinyl siloxane replicas. RESULTS: The results showed significantly lower volume gap for Control S.I.N (0.67±0.29 mm3) and SING (0.69±0.28 mm3) Ti-base abutments relative to OD (1.42±0.28 mm3) and EFF groups (1.04±0.28 mm3) (p<0.033), without significant difference between them (p=0.936). While gap values were homogenous in the central region, EFF presented a significantly higher marginal gap. Accordingly, the Control S.I.N and Singular Ti-base abutments showed improved volumetric and marginal fit relative to Odontofix and EFF. CONCLUSION: The method of manufacturing abutments influenced the misfit at the implant-abutment interface.

Analysis of correlation between optical and microtomography measurements of cementation space in CAD-CAM ceramic crowns.

STATEMENT OF PROBLEM: Identical computer-aided design (CAD) parameters may be used to mill a variety of ceramic materials for computer-aided design and computer-aided manufacture (CAD-CAM) crowns. Whether milled crown spacing matches the designed parameters when more than a single evaluation method is applied is unknown. PURPOSE: The purpose of this in vitro study was to correlate the stereomicroscope and the microcomputer tomography (µCT) 2-dimensional analysis of the cementation space with the replica technique in assessing 3 different ceramic materials. MATERIAL AND METHODS: The specimens were milled in lithium disilicate, resin nanoceramic, and zirconia (n=30). The cement space was measured at the marginal, axial, and occlusal regions. One hundred twenty measurements of each material were used to correlate the methodologies. Data were statistically evaluated with 2-way repeated measures ANOVA and the Tukey test (α=.05). The Pearson correlation coefficient was used for each region for both methodologies (µCT and optical microscopy) separately. RESULTS: For the µCT analysis, no differences were observed (P>.05) among the materials, but the axial region showed a positive correlation with the marginal (r=0.957) and occlusal regions (r=0.349); the same was observed between the occlusal and marginal regions (r=0.338). However, for stereomicroscope evaluation, resin nanoceramic and zirconia present similar cementation space (P=960), both being different from lithium disilicate (P<.05). The marginal region presented a positive correlation with axial (r=0.149) and occlusal regions (r=0.344), but the axial region showed negative correlation with the occlusal surface (r=-0.205). CONCLUSIONS: The measurements of the scanned replicas were accurate when thinner sections were under measurement. Although the same space parameters were set in the CAD software program, definitive internal dimensions varied among the milled crowns.

Three-dimensional misfit between Ti-Base abutments and implants evaluated by replica technique

Abstract An important factor affecting the biomechanical behavior of implant-supported reconstructions is the implant-abutment misfit. Objective: This study evaluated the misfit between Ti-Base abutments and implants by means of polyvinyl siloxane replica technique using microcomputed tomography (μCT). Methodology: Volumetric and linear (central and marginal) gaps of four Ti-base abutments (n=10/group): (i) Odontofix LTDA (OD), (ii) Singular Implants (SING), (iii) EFF Dental Components (EFF), and (iv) Control Group (S.I.N implants) compatible with an implant system (Strong SW, S.I.N Implants) were measured using μCT reconstructed polyvinyl siloxane replicas. Results: The results showed significantly lower volume gap for Control S.I.N (0.67±0.29 mm3) and SING (0.69±0.28 mm3) Ti-base abutments relative to OD (1.42±0.28 mm3) and EFF groups (1.04±0.28 mm3) (p<0.033), without significant difference between them (p=0.936). While gap values were homogenous in the central region, EFF presented a significantly higher marginal gap. Accordingly, the Control S.I.N and Singular Ti-base abutments showed improved volumetric and marginal fit relative to Odontofix and EFF. Conclusion: The method of manufacturing abutments influenced the misfit at the implant-abutment interface.

Influence of the composition and shades of ceramics on light transmission and degree of conversion of dual-cured resin cements.

OBJECTIVE: Since the transmittance of ceramics can influence the degree of conversion (DC) of resin cements, ceramics composition and shade should be considered in the selection of resin cement. This in vitro study aimed to evaluate the effect of the transmittance of different composition, opacities and shades of ceramics on the degree of conversion of two dual-cured resin cements. METHODOLOGY: Sixty discs were prepared from low translucency (LT) and medium opacity (MO) lithium disilicate ceramic, and zirconia ceramic (Z). Each group was subdivided into 5 subgroups (n=4) in shades A2, A3.5, B2, C2 and D3. The transmittance measurement was performed in a spectrophotometer. The Variolink II and Rely X U200 resin cements were photoactivated by LED (1400 mW/cm2) for 40 s through the ceramic discs and without the discs (control group). The DC was measured with infrared FTIR spectroscopy, immediately after light activation. Data were analyzed with Kruskall-Wallis and one-way ANOVA, following post-hoc comparisons by Tukey test and Pearson's correlation test (P<0.05). RESULTS: LT ceramic exhibited higher transmittance values compared to MO and Z ceramics. LTA2 and LTB2 showed statistically higher transmittance values compared to MOA2, MOA3.5 and ZA3.5. For Variolink II, the ceramic interposition did not influence the DC, since there were no statistical differences between groups with ceramic interposition and the control group. For Rely X U200 cement, the interposition of some ceramics types/shades (LTA3.5, MOA2, MOA3.5 and ZA3.5) significantly decreased the DC values compared to control group. A positive correlation was found between the ceramic transmittance and DC values of both tested cements. Conclusions. The transmittance and DC values of the cements were influenced by composition and shades of the ceramics. The higher the transmittance of ceramics, the higher the DC values for both cements.

The Effect of CAD/CAM Crown Material and Cement Type on Retention to Implant Abutments.

PURPOSE: To evaluate the pullout resistance of CAD/CAM implant-supported crowns cemented with provisional and definitive cements on Ti-base implant abutments. MATERIALS AND METHODS: Sixty crowns were milled for use in Ti-base implant abutments and divided (n = 15/group) according to material, as follows: (a) [Pr] Temporary acrylic resin; (b) [Co-Cr] Cobalt-Chromium alloy; (c) [Zr] polycrystalline zirconia; and (d) [Ti] titanium. The cementation was performed with RelyX Temp NE (RxT) cement or RelyX U200 self-etching resin cement, under a 50 N (5 kg) load for 10 minutes. Twenty-four hours after cementation, the crowns were subjected to the pullout test in a universal test machine, at a 1.0 mm/min crosshead speed. The tests were performed first without cement to evaluate frictional resistance (Baseline), then with provisional cement (RelyX Temp NE without cement again (Baseline After RxT), and finally with resin cement (U200). The results were analyzed by ANOVA and Tukey test (p < 0.05). RESULTS: Data evaluation as a function of cement type demonstrated the superiority of resin-based cements relative to provisional and baseline groups (p < 0.01). While Co-Cr crowns presented the highest pullout strength values, Pr showed the lowest values (data collapsed over cement) (p < 0.001). Retentiveness data as a function of both factors demonstrated similar pullout resistance between groups without cement (p < 0.001), except Zr baseline. Also, Co-Cr presented higher pullout strength compared to other materials. CONCLUSIONS: Self-adhesive resin cement exhibited superior retention compared to temporary cement, regardless of crown material. Co-Cr and titanium presented higher levels of retention to Ti-base abutment after being cemented.

Influence of the composition and shades of ceramics on light transmission and degree of conversion of dual-cured resin cements

Abstract Objective Since the transmittance of ceramics can influence the degree of conversion (DC) of resin cements, ceramics composition and shade should be considered in the selection of resin cement. This in vitro study aimed to evaluate the effect of the transmittance of different composition, opacities and shades of ceramics on the degree of conversion of two dual-cured resin cements. Methodology Sixty discs were prepared from low translucency (LT) and medium opacity (MO) lithium disilicate ceramic, and zirconia ceramic (Z). Each group was subdivided into 5 subgroups (n=4) in shades A2, A3.5, B2, C2 and D3. The transmittance measurement was performed in a spectrophotometer. The Variolink II and Rely X U200 resin cements were photoactivated by LED (1400 mW/cm2) for 40 s through the ceramic discs and without the discs (control group). The DC was measured with infrared FTIR spectroscopy, immediately after light activation. Data were analyzed with Kruskall-Wallis and one-way ANOVA, following post-hoc comparisons by Tukey test and Pearson's correlation test (P<0.05). Results LT ceramic exhibited higher transmittance values compared to MO and Z ceramics. LTA2 and LTB2 showed statistically higher transmittance values compared to MOA2, MOA3.5 and ZA3.5. For Variolink II, the ceramic interposition did not influence the DC, since there were no statistical differences between groups with ceramic interposition and the control group. For Rely X U200 cement, the interposition of some ceramics types/shades (LTA3.5, MOA2, MOA3.5 and ZA3.5) significantly decreased the DC values compared to control group. A positive correlation was found between the ceramic transmittance and DC values of both tested cements. Conclusions. The transmittance and DC values of the cements were influenced by composition and shades of the ceramics. The higher the transmittance of ceramics, the higher the DC values for both cements.

Prótese total mandibular imediata sobre implantes cone-morse / Immediate mandibular full arch prosthesis with Morse taper connection implant: clinical case

A perda total dos dentes ainda é um problema enfrentado por grande parcela da população. Nesse sentido, a Odontologia vem desenvolvendo novos métodos para a reabilitação desses pacientes. As próteses totais mucossuportadas foram amplamente utilizadas sem apresentarem, no entanto, estabilidade e retenção satisfatórias, principalmente na mandíbula. Com os estudos desenvolvidos por Brånemark, que culminaram com o aparecimento dos implantes osseointegráveis, um novo conceito de tratamento de pacientes desdentados totais surgiu: próteses totais implantossuportadas. A partir desse conceito, modificações foram realizadas no protocolo original com o intuito de tornar o tratamento mais rápido, efetivo, barato e menos traumático. Assim, pesquisas foram desenvolvidas para viabilizar o carregamento imediato dos implantes. Outro novo conceito que busca a melhora dos resultados em reabilitações orais é o sistema cone-morse, que apresenta maior resistência mecânica, melhor distribuição e transmissão de forças ao longo do implante para o tecido ósseo, redução do gap, com diminuição da possiblidade de invasão bacteriana na interface do implante intermediário, além da redução do afrouxamento e fraturas de parafusos. Fundamentado nessas considerações, este artigo tem como objetivo apresentar um caso clínico de reabilitação com prótese total imediata implantossuportada por implantes do tipo cone-morse na mandíbula. O resultado mostrou-se bastante satisfatório tanto em relação à estética quanto à função e, principalmente, ao conforto do paciente.

The edentulous condition is still a problem faced by most of the population. In this sense, dentistry has developed new methods for the rehabilitation of these patients. Initially, mucoussupported total prostheses have been widely used however without demonstrating satisfactory stability and retention, particularly in the jaw. With the studies developed by Brånemark culminating in the appearance of modern dental implants, a new concept emerged to treat edentulous patients: total implant-supported fixed prosthesis. From this concept, modifications were made in the original protocol in order to make the treatment faster, effective, inexpensive, and less traumatic. Thus, research has been developed to enable the immediate implant activation. Another new concept that aims to provide better results in the oral rehabilitation is the Morse taper system that has greater mechanical strength, better force distribution and transmission along the implant to the bone tissue, reducing the gap with less chance of bacterial invasion in the prosthetic-implant interface, and still with less loosening and screw fractures. Based on these considerations, this article aims to present a case of an immediate implant-supported mandibular total prosthesis on Morse taper implants. The result was quite satisfactory both in relation to aesthetics, function, but especially for patient's comfort.

Avaliação clínica dos fatores etiológicos das lesões cervicais não cariosas / Clinical evaluation of etiological factors of noncarious cervical lesions

O propósito desta pesquisa foi investigar a influência de diferentes fatores etiológicos na formação das lesões cervicais não cariosas (LCNCs). Essas lesões foram identificadas e classificadas de acordo com o grau de severidade e correlacionadas com os seguintes fatores: presença/ausência de facetas de desgaste e de biofilme, idade, gênero, hábitos alimentares, história médica, hábitos de higiene oral e hábitos parafuncionais. Foram avaliados 33 estudantes de graduação e pós-graduação, com idade variando entre 18 e 30 anos. A avaliação clínica constou de aplicação de índice de biofilme dental, exame clínico para identificação de LCNCs e de modelos de gesso para identificação de facetas de desgaste e aplicação de questionário. Os resultados mostraram que 25 indivíduos (75,7%) apresentaram, pelo menos, uma LCNC, com maior prevalência nas faces vestibulares, sendo os primeiros molares com maior número de lesões, seguidos pelos primeiros pré-molares; os incisivos foram os dentes com menor número de lesões; os pré-molares foram os dentes que apresentaram lesões com maiores escores de severidade; encontrou-se correlação direta entre a severidade das lesões e a idade (p=0,04; r=0,350) e entre facetas de desgaste e lesões (p<0,05; r=0,605). Dos 250 dentes com lesões cervicais, 217 (86,8%) apresentaram facetas de desgaste e 33 (13,2%) não apresentaram facetas, o que sugere a existência de relação significativa entre a presença de facetas e de lesões (p<0,05); foi encontrada, também, correlação inversa entre a presença de biofilme e o número e a severidade das lesões (p=0,02,r=-0,403; p=0,02; r=-0,426, respectivamente). Os fatores gênero, hábitos alimentares, história médica, hábitos de higiene oral e hábitos parafuncionais não foram diferenciais com relação à presença de LCNCs. Os resultados obtidos reforçam a teoria multifatorial para a etiologia dessas lesões.

The purpose of this research was to investigate the influence of different etiological factors in the formation of noncarious cervical lesions (NCCLs). These lesions were identified and classified according to the degree of severity and correlated with the following factors: presence/absence of wear facets and biofilm, age, gender, dietary habits, medical history, oral hygiene habits and parafunctional habits. Thirty-three undergraduate and graduate students were evaluated, with ages ranging from 18 to 30 years. Clinical evaluation consisted of dental biofilm index application, clinical examination to identify NCCLs and stone casts for identification of wear facets and questionnaire. The results showed that 25 individuals (75.7%) had at least one NCCL, with higher prevalence in buccal surfaces, the first molars with the higher number of lesions, followed by the first premolar; incisors were teeth with fewer lesions; premolars were teeth that had lesions with higher severity scores; direct correlation was found between severity of lesions and age (p=0.04, r=0.350), and between wear facets and lesions (p<0.05, r= 0.605); from 250 teeth with cervical lesions, 217 (86.8%) had wear facets and 33 (13.2%) had no facets, which suggests the existence of a significant relationship between the presence of facets and NCCLs (p<0.05); inverse correlation was found between the presence of plaque and the number and severity of NCCLs (p=0.02, r=- 0.403; p=0.02, r=-0.426, respectively). Gender, dietary habits, medical history, oral hygiene habits and parafunctional habits showed no significant differences regarding to the presence of NCCLs. The results support the multifactorial theory for the etiology of these lesions.

Recobrimento radicular: deslize lateral do retalho associado ao enxerto de tecido conjuntivo / Root coverage: laterally positioned flap associate with connective tissue graft

As recessões gengivais são encontradas com bastante frequência na população, gerando, em muitos casos, incômodo estético. Sensibilidade radicular também tem sido relatada, além de maior predisposição à cárie e às lesões não cariosas de abrasão e erosão na superfície radicular desnuda. A fim de alcançar um recobrimento radicular satisfatório, muitas técnicas têm sido propostas, dentre elas os enxertos gengivais livres, enxertos gengivais pediculados ou a associação de ambos. O presente artigo teve por objetivo relatar um caso clínico de recobrimento radicular através da técnica de deslize lateral do retalho associada ao enxerto de tecido conjuntivo subepitelial. O acompanhamento clínico ao longo de um ano mostrou a ocorrência de creeping attachment.

The gingival recessions are found very frequently in the population, generating in many cases, aesthetic complaint. Root sensitivity has also been reported, beyond greater predisposition to caries and non-carious lesions of abrasion and erosion on the denuded root surface. In order to achieve satisfactory root coverage, many techniques have been proposed, among them the free gingival grafts, pedicled gingival grafts or a combination of both. This article aims to report a case of root coverage using the technique of laterally positioned flap associated with the subepithelial connective tissue graft. The one-year clinical follow-up showed the occurrence of creeping attachment.